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What is bacteriostatic water?

A plain explanation of the water used to reconstitute powders in multi-dose vials: what is in it, how it differs from other diluents and what the label asks of you.

Updated

What is in it

Bacteriostatic Water for Injection, USP is sterile, non-pyrogenic water with 0.9% (9 mg/mL) benzyl alcohol added as a bacteriostatic preservative. It contains no other active ingredient.

Bacteriostatic means it slows the growth of bacteria. It does not mean it kills everything or that the vial can be handled carelessly.

How it differs from sterile water and saline

  • Sterile water for injection has no preservative. It is usually meant for a single use and the rest is discarded.
  • Bacteriostatic water has the benzyl alcohol preservative, so a vial can be used for more than one draw within the time its label allows.
  • Sodium chloride 0.9% (saline) is salt water. Some products say on their own leaflet which diluent to use; follow that, not a general rule.

What the label asks of you

  • Write the date you first open the vial on it. A common rule for opened multi-dose vials is to discard them 28 days after first use, unless the label gives a different date.
  • Store it as the label says and do not use it if it looks cloudy or contains particles.
  • Benzyl alcohol is not used for newborns, and some people are sensitive to it. Your pharmacist can tell you if it suits you.

How the amount of water changes the numbers

The water you add changes the concentration, not the amount of the substance in the vial. More water means each unit on the syringe holds less, so the same dose takes more volume.

The reconstitution calculator turns the vial amount, the water added and your prescribed dose into units on a U-100 syringe.

Reconstitution calculator Syringe units converter

Questions

Is bacteriostatic water the same as sterile water?

No. Both are sterile, but bacteriostatic water contains 0.9% benzyl alcohol as a preservative, so a vial can be entered more than once. Sterile water for injection has no preservative.

How long does bacteriostatic water last after opening?

A common rule for opened multi-dose vials is to discard them 28 days after first use, unless the label gives a different date. Writing the date on the vial helps.

Does the amount of water change the dose?

No. It changes the concentration. The amount of the substance stays the same; you draw more or less volume for the same dose.

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